Effect of salts on retention in hydrophilic interaction chromatography
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چکیده
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15 صفحه اولHydrophilic interaction chromatography
In the early 1990s, hydrophilic interaction (liquid) chromatography (HILIC) emerged as a new separation method. HILIC is becoming increasingly popular for the separation of polar compounds in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). It has become important for the separation of pharmaceuticals, neurotransmitters, nucleosides, nucleotides and other compounds...
متن کامل[Systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography].
A systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography (HILIC) was performed. The influences of mobile phase, stationary phase and buffer salt on the retention of carbohydrates were investigated. According to the results, the retention time of carbohydrates decreased as the proportion of acetonitrile in mobile phase decreased. Increased t...
متن کاملHydrophilic interaction liquid chromatography (HILIC) in proteomics
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2018
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2018.01.038